streptavidin dyes (Jackson Immuno)
Jackson Immuno is a verified supplier 
96
Structured Review
Jackson Immuno
streptavidin dyes
Streptavidin Dyes, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 5444 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+dyes/Streptavidin/pmc12839970-257-82-90
Average 96 stars, based on 5444 article reviews
Streptavidin Dyes, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 5444 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+dyes/Streptavidin/pmc12839970-257-82-90
Average 96 stars, based on 5444 article reviews
streptavidin dyes - by Bioz Stars,
2026-11
96/100 stars
Images
1) Product Images from "Landscape expansion microscopy reveals interactions between membrane and phase-separated organelles"
Article Title: Landscape expansion microscopy reveals interactions between membrane and phase-separated organelles
Journal: The Journal of Cell Biology
doi: 10.1083/jcb.202502035
Figure Legend Snippet: land-ExM visualizes phase-separated and membrane organelles. (A–G) land-ExM protein images of membraneless phase separation structures. The proteins were labeled with NHS-biotin-MS and after gelation stained with streptavidin-Alexa Fluor 488. (A) land-ExM protein image of nucleoli in a U2OS cell. Red arrowheads indicate the fibrillar center (FC) or dense fibrillar component (DFC) of the nucleolus. Scale bar: 1 µm in pre-expansion unit. Linear expansion factor: 4.0. (B) land-ExM protein image of nuclear bodies of breast cancer cell, UCI082014. Red arrowheads indicate the nuclear bodies. Scale bar: 1 µm in pre-expansion unit. Linear expansion factor: 4.2. (C) land-ExM protein image of SGs of a U2OS cell treated with NaAsO 2 for 20 min. The red arrowhead indicates a SG. Scale bar: 1 µm in pre-expansion unit. Linear expansion factor: 4.0. (D) land-ExM protein image of chromatin of a breast cancer cell. Scale bar: 500 nm in pre-expansion unit. Linear expansion factor: 4.2. (E) land-ExM protein image of NPCs of a breast cancer cell. Scale bar: 1 µm in pre-expansion unit. Linear expansion factor: 4.2. (F and G) land-ExM protein images of mitochondria and cytoskeleton of a U2OS cell. Scale bar: 1 µm in pre-expansion unit. Linear expansion factor: 4.0. (H–P) land-ExM lipid images of membrane structures. The lipids were labeled with mCLING-Atto647N. (H) land-ExM lipid image of breast cancer cell. Scale bar: 5 µm in pre-expansion unit. Linear expansion factor: 4.0. (I–M) magnified images of H showing different membrane structures: lipid vesicles (I), mitochondria (J), filopodia (K), nuclear invagination (L), and Golgi apparatus (M). Scale bar: 1 µm (I–M) in pre-expansion unit. (N) 3D land-ExM lipid image of a breast cancer cell after maximum intensity projection, showing the cell membrane. Color-coded by the z-dimension slices from bottom to top. Color bar: purple to white: 0–6 µm in pre-expansion unit. Scale bar: 5 µm in pre-expansion unit. Linear expansion factor: 4.0. (O and P) magnified images of N showing detailed structures of the cell membrane. Scale bar: 1 µm in pre-expansion unit. All images were taken with an Airyscan microscope.
Techniques Used: Membrane, Labeling, Staining, Microscopy
Related Articles
Incubation:Article Title: Landscape expansion microscopy reveals interactions between membrane and phase-separated organelles. Article Snippet: Antibody LR-ExM anti-FITC-phalloidin antimouse two color kit Cytoseen LR-RBF-MD025 G3BP1 antibody (TT-Y) Santa Cruz Biotechnology sc-81940 Sec61b (D5Q1W) Rabbit mAb Cell Signaling Technology 14648 Anti-TOMM20 antibody [EPR1558154] Abcam ab186735 LAMP-2 (H4B4) Santa Cruz Biotechnology sc-18822 Anti-clathrin heavy chain antibody Abcam ab21679 Fluorescein Phalloidin Invitrogen F432 Fluorescein/Oregon Green Polyclonal Antibody, Alexa Fluor 488 Invitrogen A11096 LR-ExM anti-rabbit antibodydigoxigenin trifunctional anchor and anti-digoxigenin dye kit Cytoseen LR-RD488050 LR-ExM anti-mouse antibodydigoxigenin trifunctional anchor and anti-digoxigenin dye kit Cytoseen LR-MD568050 AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResearch 111-005-144 AffiniPure Goat Anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-005-166 DyLight 405 Streptavidin Jackson ImmunoResearch 016-470- 084 Alexa Fluor 488 Streptavidin Jackson ImmunoResearch 016-540- 084 Protein and lipid labeling reagents land-ExM NHS-biotin-MA and Streptavidin-488 kit Cytoseen LD-P488050 land-ExM NHS-biotin-MA and Streptavidin-405 kit Cytoseen LD-P405050 mCLING-Atto 647N Synaptic Systems 710 006AT647N Alexa Fluor 488 NHS Ester (Succinimidyl ester) Invitrogen A20000 Alexa Fluor 568 NHS Ester (Succinimidyl ester) Invitrogen A20003 Other reagents and supplies SYLGARD 184 Silicone Elastomer Kit Ellsworth 2137054 12-mm, no. 1 cover glass Bellco Glass 1943– 10012A Paraformaldehyde Electron Microscopy Sciences 50980488 Formaldehyde Sigma-Aldrich F8775 Aqueous glutaraldehyde EM Grade 10% Electron Microscopy Sciences 16120 Table 1. .. The cell-gelation solution was then incubated at 37°C for 1–2 h. Gelled cells were immersed in heat denaturation buffer (200 mM sodium dodecyl sulfate, 200 mM NaCl, and 50 mM Tris, pH 6.8) for 1.5 h at 78°C and washed with excess of water for 30 min. To fluorescently label the whole proteins, immerse the gelled cells in poststaining buffer (10 mM HEPES and 150 mM NaCl, pH 7.5) twice, 30 min each time, then incubate the gelled cells with 4 μg/ml Article Title: Landscape expansion microscopy reveals interactions between membrane and phase-separated organelles Article Snippet: .. The cell-gelation solution was then incubated at 37°C for 1–2 h. Gelled cells were immersed in heat denaturation buffer (200 mM sodium dodecyl sulfate, 200 mM NaCl, and 50 mM Tris, pH 6.8) for 1.5 h at 78°C and washed with excess of water for 30 min. To fluorescently label the whole proteins, immerse the gelled cells in poststaining buffer (10 mM HEPES and 150 mM NaCl, pH 7.5) twice, 30 min each time, then incubate the gelled cells with 4 μg/ml |